Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?

DAPI-fluorescent fading: a problem in microscopy or a way to measure nuclear DNA content?

In observation by confocal or conventional fluorescence microscopy, the retardation of the lost in fluorescence, from highest signal of fluorescence to lowest intensity are important factors in order to obtain accurate images. This problem is very common in fluorochromes for nuclear DNA and especially for DAPI stain. The fluorescence of DAPI is rapidly lost when it is exposure to excitation by ...

Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes.

We discuss the use of fluorescence correlation spectroscopy for the measurement of relatively slow rotations of large macromolecules in solution or attached to other macromolecular structures. We present simulations and experimental results to illustrate the range of rotational correlation times and diffusion times that the technique can analyze. In particular, we examine various methods to ana...

investigating the relationship between ambiguity tolerance and willingness to translate of iranian prospective english translators: an sem revalidation study

cognitive studies of translation process have recently been awarded a great deal of attention. there exists a psychological angle to almost all translation activities. the present study, thus, deals with analysing the relationships between iranian prospective translators tolerance for ambiguity (ta) and their willingness to translate (wtt). the research was conducted as a mixed methods study, d...

Structural investigation of complexes formed by DNA+CTAB and DNA+DDAB and Designing a method to increase salt ions between DNA and the Surfactant rods.

The internal structure of DNA-CTAB and DNA-DDAB is investigated by a Small Angel X- ray Scattering(SAXS) instrument. Hexagonal packing of DNA was observed for DNA complex with CTAB, and for DDABcomplex is observed lamellar structure. Variations in the internal spacing and degree of long-range ordering aredependent on both surfactant type and concentrations of added salt. When we increased the a...

The Relationship between Dna Content and Alternative Meiotic Patterns in Certain Discocephalinids (pentatomidae; Heteroptera)